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Image Search Results
Journal: The Journal of experimental biology
Article Title: Purification and characterisation of endo-beta-1,4-glucanase and laminarinase enzymes from the gecarcinid land crab Gecarcoidea natalis and the aquatic crayfish Cherax destructor.
doi: 10.1242/jeb.017657
Figure Lengend Snippet: Fig. 4. SDS polyacrylamide gel electrophoresis of purified and partially purified endo-β-1,4-glucanase and laminarinase from the midgut gland of C. destructor and G. natalis. Gels were silver stained. (A) Lane 1, molecular mass standards with their sizes indicated in kDa. Lane 2, endo- β-1,4-glucanase purified from the midgut gland of G. natalis. Lane 3, endo- β-1,4-glucanase 2 purified from the midgut gland of C. destructor. Lane 4, laminarinase purified from the midgut gland of G. natalis. Lanes 5 and 6, laminarinases partially purified from midgut gland of C. destructor using either Bio-Rad P-100 gel filtration chromatography (Lane 5) or Mono-Q strong anion chromatography (Lane 6) as the third purification step. (B) Lane 1, endo-β-1,4-glucanase 1 purified from the midgut gland of C. destructor. Lane 2, molecular mass standards with their masses indicated in kDa.
Article Snippet: Gel filtration chromatography Protein concentrates from either the anion exchange chromatography step or the HIC step were applied to a 1.5cm 50cm P-100 medium
Techniques: Polyacrylamide Gel Electrophoresis, Purification, Staining, Filtration, Chromatography